Posts

EUTHANASIA PROCEDURES USED IN EXPERIMENTAL ANIMALS

-
Image
In experimental pharmacology, animals need to be sacrificed to obtain different organs for evaluating drug activity. Euthanasia is used where an animal is required to be sacrificed on termination of an experiment or otherwise for ethical reasons. There are various procedures carried out by which an animal is sacrificed quickly and painlessly in an atmosphere free from fear or anxiety. For accepting an euthanasia method as humane, it should have an initial CNS depressive action for immediate insensitivity to pain. The choice of a method will depend on the nature of the study and the species of animal to be killed. Euthanasia methods are classified into chemical and physical methods. Chemical method: different chemicals, due to overdose or their toxic effect, may cause death, but very few are recommended for euthanasia. Chemical methods may be inhalants or non-inhalants. Inhalant methods: Inhalant anaesthetics (nitrous oxide, ether, halothane, enflurane, sevoflurane, methoxyflurane,...
Post a Comment
Read more

ANAESTHETICS USED IN EXPERIMENTAL ANIMALS

-
Image
Anaesthesia used in the experimental study is to ensure analgesia, amnesia and immobilization of animals during the procedure. Unless contrary to the achievement of the results of the study, different anaesthetics should be used to control pain or distress during the experiment, making the study simpler, reliable and reproducible. The painful procedures are conducted under appropriate anaesthesia as recommended for each species of animal.   It must also be ensured that the anaesthesia is given for the full duration of the experiment, and at no stage is the animal conscious to perceive pain. Local or general anaesthesia may be used, depending on the type of surgical procedure. Local anaesthetics are used to block the nerve supply to a limited area and are used only for minor and rapid procedures. This should be carried out under expert supervision for regional infiltration of the surgical site, nerve blocks, and for epidural and spinal anaesthesia. The most common compounds for surf...
Post a Comment
Read more